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Liporeductyl and Tri-peptide 1This ingredient - Tripeptide-1 - is a specialized molecule that is a synthetic peptide, containing three amino acid residues - glycerine, histidine and lysine. It is a patented ingredient made by Lipotec in Spain and is classified as a protein derivative. It is used as a miscellaneous skin-conditioning agent.
It is one of the active ingredients contained in Cellumend Anti-Cellulite Cream, and has been clinically tested and evaluated for its effectiveness. It is combined with the other active ingredients in the cream, to work together in an unique synergistic way, making it impossible for competitors to duplicate the amazing results that we achieve with this cream. To help visitors understand the action of our cellulite cream better, we have included the clinical test results below, on this page, of both the ‘in vitro’ and ‘in vivo’ test conducted on Liporeductyl. The test period, at the University of Milan, was taken over a 60-day period, performed on twenty females aged between 18 and 70 years of age, all with cellulite problems.
Cellulite prevention
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Active ingredient at 0 µg/ml | |
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Active ingredient at 0.1 µg/m |
Active ingredient at 1 µg/m | |
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Active ingredient at 10 µg/m |
Active ingredient at 100 µg/m | |
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Although the active ingredient was developed to remove and prevent cellulite, some interesting and very positive effects were found when the clinical trials were done.
Skin aging is accelerated by normal processes, such as lipid peroxidation. It has been found that tissues affected by skin aging contain higher and elevated levels of reactive A,ß-unsaturated aldehydes, such as 4-Hydroxy-2-nonenal(HNE), Malondialdehyde (MDA) and acrolein.
It was however found that Tripeptide GHK is a potent aldehyde sequestering agent. This is currently under patent application. This added benefit of capturing lipid peroxidation by-products, is over and above due to the positive effective effect it has on removing and preventing cellulite.
The tables below quantify the capture of lipid peroxidation byproducts
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A 3D in vitro skin model was used for the absorption studies, which consisted of pluri-stratified human keratinocytes, showing morphology and functionality close to that of in vivo human skin. In the 3D skin model, a semi-permeable membrane is found under the artificial epidermis. This membrane is in contact with the culture medium (referred to as undernatant). The skin model was constructed by Skinethic®, from the Skinethic Laboratories in Nice.
For the test purposes, 200 mg of the active ingredient was added to each epidermal unit, with the quantities of caffeine and that of escin previously determined.
Pure caffeine was diluted to 20 ppm in the culture medium and 200 µL was placed in contact with the skin model.
Pure escin was diluted to 50 ppm in the culture medium and 200 µL was placed in contact with the skin model.
200 µL samples of the undernatant were taken at 2, 4 and 16 hours to evaluate the caffeine and escin absorption. All the samples were weighed and analyzed by HPLC.
Caffeine versus Cellumend formula
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Escin versus Cellumend formula
Conclusion
From the absorption tests done, it can clearly be seen that the liposomal formulation used with the Cellumend cream favors epidermal penetration - this fact is clearly demonstrated: that penetration of the formulation is almost 5 times that of pure caffeine and almost 8 times that of pure escin.
The way that the formulation encapsulates the active ingredients in liposomes also enables slow release, as proved by the determination of the 16 hour test, where the formula is absorbed more than 3 times than that of pure caffeine and 7.7 times than that of pure escin.
The measurements were taken on specific body areas:
All instruments used in the tests are authorized according to EU (European Union) regulations.
The following were tested: Circumference of buttocks and thighs, body fat mass (using STA/BIA instrument), extra cellular water (STA/BIA instrument), skin moisture (using a corneometer) and elasticity (using a cutometer).
The graph below indicates the reduction in buttock size - in 15% of the patients a very significant decrease of 2.0 - 3.0 cm was achieved, while 60% showed a decrease of 0.5 - 1.0 cm during the test period of 60 days.
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The clinical tests showed that 85% of patients showed a significant decrease of 0.5 cm to 1.0 cm in the size of the thighs.
As shown in the graph below, an average decrease of between 0.5 liter ( 500 ml) to 1.6 liter (1,600 ml) was achieved per person in 60% of the patients being tested during the clinical trials.
It must be noted that Cellumend does NOT only reduces excess water, but works on the entire system to help reduce cellulite. Although excess water is one factor that helps to cause cellulite, it is NOT the only one, and the build-up of toxins and waste products must also be dealt with in order to effectively remove and prevent cellulite.
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The graph below shows schematically the fact that 50% of the patients that participated in the clinical tests, had a significant decrease of 0.6 to 1.4 kilogram (1.32 to 3.08 pounds) in body mass while testing the efficiency of the cellulite removing cream.
The skin elasticity was tested by means of a cutometer and it was found that an average increase of 17.5% elasticity was achieved after 30 days, and an average increase of 24.4% after the 60 day testing period.
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From the graph a person can see that the moisturizing index of the skin, during the clinical tests, increased by an average of 9.5%.
A score between 1 - 4 was given to the dermatologist's evaluation and a Friedman test was used for the statistical analysis of this non-parametric data. (Conover, W. J. 1980. Practical Nonparametric Statistics. 2nd ed. New York: John Wiley & Sons.; Daniel, Wayne W. 1978. Applied Nonparametric Statistics. Boston: Houghton Mifflin.; Daniel, Wayne W. 1995. Biostatistics. 6th ed. New York: John Wiley & Sons.; Hollander, M. and Wolfe, D. A. 1973. Nonparametric Statistical Methods. New York: John Wiley & Sons.)
As shown in the graph below, it was shown that the polygonal net was more regular in 60% of the patients.
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As shown in the graph below, patients had an improvement on the orange peel skin effect experienced in cellulite.
The graph below shows that in 40% of the patients, the nodule condition improved and both micro-nodules and macro-nodules seemed less evident.
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As shown in the graph, 65% of the patients finished the test with far smoother skin.
The graph below shows that there was a significant variation of skin compactness from the 30th day of treatment, which was an index of improved cutaneous tropism. After the 60 day treatment 55% of the patients showed a compact and elastic skin.
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After the tests 65% of the patients showed a rosier complexion, which is a sign of improved microcirculation.
During the clinical evaluation, video capillaroscopy was used.
Images of the skin were taken, before and after the test. These images were made using 200 X magnification on cellulite imperfections, thereby allowing the study of microcirculation.
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Liporeductyl is used in the following of our products |
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