Serilesine^® - stimulation of fibroblast creation

Different clinical tests were done to prove that Serilesine helps to keep skin aging at bay by stimulating various mechanisms.

This page looks at how it helps to stimulate the formation of fibroblast in the skin.

To place this in context with how it helps to fight aging and to have a younger looking skin, please look at the Serilesine summary page by clicking here.

In vitro fibroblast proliferation

• For more on fibroblast please click here.

Cell viability is evaluated through an MTT assay. The key component is 3-[4,5dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide or MTT, which is yellowish in solution.

Mitochondrial dehydrogenases of viable cells cleave its tetrazolium ring, leading to the formation of purple crystals which are insoluble in aqueous solution.

The crystals are re-dissolved in acidified isopropanol and the color in the resulting purple solution is quantified spectrophotometrically.

An increase or decrease in cell number results in a proportional change in the amount of formazan formed, indicating the degree of inhibiting or stimulating cell growth.

Trypsinised fibroblast from human skin are incubated for 48 hours in microplates with SERILESINE® (0.05 mg/ml), Minimal Essential Medium-Na-Piruvate and 5% Fetal calf Serum (FCS).

After the incubation with the tested substance and after medium substitution with fresh medium and MTT, cells are incubated for 3 hours at 37 ºC. Then cells are washed to eliminate MTT excess. The fluorimeter reading is performed at 540 nm wavelength.

A culture of fibroblasts was treated with 0.05 mg/ml SERILESINE® and results were measured after 48 hours. Results are given as optical density (O.D.), that is proportional to cell number:

After 48 hours an increase of 74.6% was noted in fibroblast proliferation.

Due to the fact that combining all the images on one page makes it too large to download within a fair period of time, we created different pages dealing with each aspect that was tested.

The other test that were performed can be viewed by clicking the hyperlink.

• Serilesine increases Laminin (I)
• Serilesine increases Laminin (II)
• Serilesine increases a6-Integrin production
• Serilesine stimulates hemidesmosome formation
• Serilesine increases keratinocyte bonding
• Serilesine increases keratinocyte creation
• Serilesine increase the creation of fibroblast
• Serilesine increases skin elasticity
• Serilesine increases skin compactness (firmness)
• Serilesine increases skin tone
• Serilesine increases skin smoothness

If you are looking for the summary page of Serilesine please click here.