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Different clinical tests were done to prove that Serilesine helps
to keep skin aging at bay stimulating various mechanisms.
This page looks at how it helps to boost the production of
laminin.
To place this in context with how it helps to fight aging and to have a younger looking skin, please look at the Serilesine summary page by clicking here.
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Serilesine is used in the following products: |
Immunostaining studies have been carried out in human fibroblasts (the natural producers of Laminin) in order to ascertain if SERILESINE® is able to enhance the expression of Laminin-5.
The test uses a primary (monoclonal) antibody that binds to Laminin-5, and a secondary antibody (polyclonal) that binds to the complex between protein and
the primary antibody. The secondary antibody is coupled to a fluorescent compound (FITC – Fluorescein Isothiocyanate).
FITC is illuminated with filtered light at 495 nm (absorption wavelength) and the light emitted by the dye is detected at 528 nm (emission wavelength).
Trypsinised fibroblasts were incubated for 72 hours in microplates with 0.05 mg/ml SERILESINE®, Minimal Essential Medium-Na-Piruvate and 5% FCS.
Cells were then fixed and incubated with the primary antibody and then with the secondary antibody coupled to the dye (FITC). For the immunofluorescence analysis we used an Olympus fluorescence microscope (20x magnification).
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 | Untreated human fibroblast | Treated human fibroblast |
Due to the fact that combining all the images on one page makes it too large to download within a fair period of time, we created different pages dealing with each aspect that was tested.
The other test that were performed can be viewed by clicking the hyperlink.
If you are looking for the summary page of Serilesine please click here.
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